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Estimated Number of Cells

6,945

Post-Normalization Mean Reads per Cell

47,114

Median Genes per Cell

1,468
Pre-Normalization Number of Reads
Total number of read pairs that were assigned to these libraries in demultiplexing.
Post-Normalization Number of Reads
Number of read pairs after normalizing for depth among multiple libraries.

Sequencing

Pre-Normalization Number of Reads 374,204,407
Post-Normalization Number of Reads 327,213,233
library 0 Fraction of Reads Kept
The fraction of reads kept for this input sample, after normalizing depth across samples to reduce batch effects.
library 1 Fraction of Reads Kept
The fraction of reads kept for this input sample, after normalizing depth across samples to reduce batch effects.
library 0 Pre-Normalization Total Reads per Cell
The mean number of total sequencing reads per cell in this input sample, prior to depth normalization.
library 1 Pre-Normalization Total Reads per Cell
The mean number of total sequencing reads per cell in this input sample, prior to depth normalization.
library 0 Pre-Normalization Confidently Mapped Barcoded Reads per Cell
The mean number of confidently-mapped-to-transcriptome, valid-barcode reads per cell in this input sample, prior to depth normalization.
library 1 Pre-Normalization Confidently Mapped Barcoded Reads per Cell
The mean number of confidently-mapped-to-transcriptome, valid-barcode reads per cell in this input sample, prior to depth normalization.

Aggregation

library 0 Fraction of Reads Kept 74.8%
library 1 Fraction of Reads Kept 100.0%
library 0 Pre-Normalization Total Reads per Cell 67,677
library 1 Pre-Normalization Total Reads per Cell 44,831
library 0 Pre-Normalization Confidently Mapped Barcoded Reads per Cell 35,725
library 1 Pre-Normalization Confidently Mapped Barcoded Reads per Cell 26,708
 
Estimated Number of Cells
The total number of barcodes associated with cell-containing partitions, estimated from the barcode count distribution.
Fraction Reads in Cells
The fraction of valid-barcode, confidently-mapped-to-transcriptome reads with cell-associated barcodes.
Pre-Normalization Mean Reads per Cell
The total number of sequenced reads divided by the number of barcodes associated with cell-containing partitions.
Post-Normalization Mean Reads per Cell
The total number of (depth-normalized) reads divided by the number of barcodes associated with cell-containing partitions.
Median Genes per Cell
The median number of genes detected per cell-associated barcode. Detection is defined as the presence of at least 1 UMI count.
Median UMI Counts per Cell
The median number of UMI counts per cell-associated barcode.

Cells

Estimated Number of Cells 6,945
Fraction Reads in Cells 86.2%
Pre-Normalization Mean Reads per Cell 53,881
Post-Normalization Mean Reads per Cell 47,114
Median Genes per Cell 1,468
Median UMI Counts per Cell 4,158

Sample

Name JP8JP9
Description
Transcriptome mm10
Chemistry Single Cell 3' v2
Cell Ranger Version 2.2.0

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